|
|
|
|
|
| Favreau, Philippe; Benoit, Evelyne; Hocking, Henry G.; Carlier, Ludovic; D'Hoedt, Dieter; Leipold, Enrico; Markgraf, Rene; Schlumberger, Sebastien; Cordova, Marco A.; Gaertner, Hubert; Paolini-bertrand, Marianne; Hartley, Oliver; Tytgat, Jan; Heinemann, Stefan H.; Bertrand, Daniel; Boelens, Rolf; Stoecklin, Reto; Molgo, Jordi. |
| BACKGROUND AND PURPOSE The mu-conopeptide family is defined by its ability to block voltage-gated sodium channels (VGSCs), a property that can be used for the development of myorelaxants and analgesics. We characterized the pharmacology of a new mu-conopeptide (mu-CnIIIC) on a range of preparations and molecular targets to assess its potential as a myorelaxant. EXPERIMENTAL APPROACH mu-CnIIIC was sequenced, synthesized and characterized by its direct block of elicited twitch tension in mouse skeletal muscle and action potentials in mouse sciatic and pike olfactory nerves. mu-CnIIIC was also studied on HEK-293 cells expressing various rodent VGSCs and also on voltage-gated potassium channels and nicotinic acetylcholine receptors (nAChRs) to assess... |
| Tipo: Text |
Palavras-chave: Cone snail venom; Mu-conotoxin; Voltage-gated sodium channel; Nicotinic acetylcholine receptor; Myorelaxant; Twitch tension; NMR structure. |
| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00467/57830/60132.pdf |
| |
|
|
| Hess, Philipp; Abadie, Eric; Herve, Fabienne; Berteaux, Tom; Sechet, Veronique; Araoz, Romulo; Molgo, Jordi; Zakarian, Armen; Sibat, Manoella; Rundberget, Thomas; Miles, Christopher O.; Amzil, Zouher. |
| Following a review of official control data on shellfish in France, Ingril Lagoon had been identified as a site where positive mouse bioassays for lipophilic toxins had been repeatedly observed. These unexplained mouse bioassays, also called atypical toxicity, coincided with an absence of regulated toxins and rapid death times in mice observed in the assay. The present study describes pinnatoxin G as the main compound responsible for the toxicity observed using the mouse bioassay for lipophilic toxins. Using a well-characterised standard for pinnatoxin G, LC-MS/MS analysis of mussel samples collected from 2009 to 2012 revealed regular occurrences of pinnatoxin G at levels sufficient to account for the toxicity in the mouse bioassays. Baseline levels of... |
| Tipo: Text |
Palavras-chave: Cyclic imines; Shellfish toxin; Accumulation; Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS); Unexplained mouse toxicity. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00158/26960/25255.pdf |
| |
|
|
| Hess, Philipp; Herve, Fabienne; Abadie, Eric; Sechet, Veronique; Molgo, Jordi; Amzil, Zouher; Fessard, Valerie. |
| Suite à la découverte de la Pinnatoxine G comme agent responsable des toxicités atypiques à Ingril fin 2010, une étude a été commanditée afin d’approfondir les connaissances sur l’organisme producteur de cette toxine, Vulcanodinium rugosum, les toxines associées et leur toxicité. L’organisme, étant considéré « cryptique » dû à sa faible présence dans le milieu, a été suivi de manière plus proche à Ingril. Il est apparu dans la colonne d’eau en juillet et août 2012, et quelques échantillons ont pu être prélevés pour la mise en culture de souches supplémentaires. Une corrélation directe entre la croissance de l’organisme et la température est soupçonnée mais n’a pas encore pu être corroborée sur la période d’étude, faute d’un nombre suffisant... |
| Tipo: Text |
|
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00094/20518/18190.pdf |
| |
|
|
|
